Detect accessible chromatin using ATAC-sequencing, from principle to applications
نویسندگان
چکیده
منابع مشابه
Mapping accessible chromatin regions using Sono-Seq.
Disruptions in local chromatin structure often indicate features of biological interest such as regulatory regions. We find that sonication of cross-linked chromatin, when combined with a size-selection step and massively parallel short-read sequencing, can be used as a method (Sono-Seq) to map locations of high chromatin accessibility in promoter regions. Sono-Seq sites frequently correspond t...
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This unit describes Assay for Transposase-Accessible Chromatin with high-throughput sequencing (ATAC-seq), a method for mapping chromatin accessibility genome-wide. This method probes DNA accessibility with hyperactive Tn5 transposase, which inserts sequencing adapters into accessible regions of chromatin. Sequencing reads can then be used to infer regions of increased accessibility, as well as...
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Flexibility and adaptivity are two of the outstandig characteristics of new media and new technologies in learning, teaching and research. These properties allow new methods to provide physically challenged people with appropriate education. Accessibility of Web applications is typically focused on the perception and control of information. In contrast, the transfer of knowledge as complex info...
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ژورنال
عنوان ژورنال: Hereditas
سال: 2019
ISSN: 1601-5223
DOI: 10.1186/s41065-019-0105-9